Summary
The project will develop tools to enable genetic manipulation of bacteria to facilitate an array of biotechnology applications. The tools build on established methods to influence the production of protein from individual bacterial genes, including scaling up to impact all the genes in a bacterium within a single test tube. Furthermore, advances to the technology will expand to enable more precise alterations to turn genes “down” or “up,” decreasing or increasing production of proteins encoded by those genes on a broad scale. Previously, such approaches were limited to a small number of target
