Summary
Abstract JC polyomavirus (JCPyV) is the causative agent of the often-fatal demyelinating brain disease Progressive multifocal leukoencephalopathy (PML). While 70-90% of adults carry JCPyV lifelong, only after immunosuppression is the virus able to travel from the primary infection reservoir in the urinary tract to the central nervous system. How the virus traffics to and enters the brain is unknown. Utilizing the mouse polyomavirus (MuPyV), a natural mouse pathogen, I have employed Fluorescence-activated cell sorting (FACS), RT-qPCR, flow cytometry, and immunofluorescent imaging to phenotype a
