Summary
The collection of active proteins in the body contains vast information that can improve disease understanding and diagnosis. However, many disease-related proteins exist at very low levels and are difficult to detect. Furthermore, many detection tools measure protein abundance but not protein function. This CAREER project will develop ultrasensitive tools to measure simultaneously low abundance proteins and their activities. The project will build on a recently developed single-molecule protein detection technology that enables protein measurements at concentrations far below the limits of co
